Leukotriene D4 Induces Production of Transforming Growth Factor-β1 by Eosinophils
| Autor: | Hitoshi Kamiya, Jun Atsuta, Kosei Iguchi, Hisashi Nishimori, Hajime Katsumata, Takao Fujisawa, Yoshiko Kato |
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| Rok vydání: | 2005 |
| Předmět: |
Leukotriene D4
Immunology General Medicine Transforming growth factor beta respiratory system Biology Granulocyte Eosinophil Allergic inflammation chemistry.chemical_compound medicine.anatomical_structure chemistry Eicosanoid medicine biology.protein Immunology and Allergy Interleukin 5 Transforming growth factor |
| Zdroj: | International Archives of Allergy and Immunology. 137:17-20 |
| ISSN: | 1423-0097 1018-2438 |
| Popis: | Eosinophils may play an important role in the pathogenesis of airway remodeling in asthma through the production of various fibrogenic cytokines such as transforming growth factor-β1 (TGF-β1). Cysteinyl leukotrienes are also suggested to be involved in remodeling with their potential to induce proliferation of airway smooth muscle cells. Since massive eosinophil infiltration and the release of cysteinyl leukotrienes in airway secretions are often seen in asthma, we hypothesized that cysteinyl leukotrienes may be involved in airway remodeling through induction of TGF-β1 from eosinophils. Peripheral blood eosinophils were cultured with leukotriene D4 (LTD4) and/or interleukin-5 (IL-5) or granulocyte colony-stimulating factor (GM-CSF) for 16 h and gene expression of TGF-β1 was quantified with real-time PCR. A combination of LTD4 and IL-5 or LTD4 and GM-CSF synergistically induced TGF-β1 expression in eosinophils although stimulation with single factor, LTD4, IL-5 or GM-CSF did not induce the gene expression. LTD4 also induced significant gene expression in eosinophils cultured in an intercellular adhesion molecule-1-coated plate. The results suggested that CysLTs stimulate eosinophils to induce TGF-β1 production in allergic inflammation where IL-5 and GM-CSF are abundant and may be involved in the pathogenesis of airway remodeling. |
| Databáze: | OpenAIRE |
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