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Objective: To evaluate the acute toxicity studies of methanolic seed extract of Tectona grandis in albino mice. Method: The present study has been carried out to evaluate the adverse and hazardous effects of methanolic seed extract of Tectona grandis in albino mice through oral route. Acute oral toxicity of methanolic extract was carried out in two phases. In first phase, a single dose of 10, 50, 100, 500 and 1000mg/kg (dissolved in water) body weight in test group, while mice in control group received normal saline was administered. All the animals were individually studies for the mortality, wellness parameters and body weight for 14 days. The experiment was performed under OECD guidelines 423. No mortality or any significant physiological changes in the animals were observed up to 1000mg/kg bodyweight. The experiment was again repeated for second phase with a single doses of 1000, 1250, 1500 and 2000 mg/kg. Mortality was found at 1250 mg/kg. Therefore LD50 of the extract was estimated to be more than 1000mg/kg body weight. Finally, administration of a single dose of 10, 50, 100, 500, 1000 mg/kg of the extract did not showed any significant differences in food and water consumption and other behavioral changes. The extract starts showing toxicity at a dose of 1250 mg/kg body weight. Conclusion: The analysis of these results with the information of signs, behavior and health monitoring leads to the conclusion that the oral administration of T.grandis methanolic seed extract for 15 days does not cause acute toxicity. But, higher doses on long term administering can cause considerable toxicity. |
