Triploidization in pikeperch (Sander lucioperca) induced by cold shock
| Autor: | Oleksandr Malinovskyi, Tomas Policar, Ievgen Lebeda, Martin Flajšhans, Miroslav Blecha, Hadiseh Dadras, Jiří Křišťan |
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| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Control treatment Hatching fungi 04 agricultural and veterinary sciences Aquatic Science Biology 03 medical and health sciences Animal science Human fertilization Shock (circulatory) 040102 fisheries medicine 0401 agriculture forestry and fisheries Effective treatment Shock treatments medicine.symptom 030304 developmental biology Shock duration |
| Zdroj: | Aquaculture. 533:736236 |
| ISSN: | 0044-8486 |
| DOI: | 10.1016/j.aquaculture.2020.736236 |
| Popis: | The present research investigated induction of triploidy using a cold temperature shock in pikeperch, Sander lucioperca L. In this study, a cold shock treatment by immersing eggs in an ice-water bath (1.0–1.1 °C) at 5, 7, 10, 15, and 20 min post-activation at fertilization (PA) for 20, 40, 60, 90, and 120 min was applied. Three out of 25 tested combinations of initiation time and shock duration resulted in 100% triploidy: initiation at 5 min PA for 90 and 120 min and at 7 min PA for 120 min. Shock treatments initiated at 20 min PA resulted in all- diploid larvae. The control treatment showed hatching rate of 66.2%, without any triploid production and 2.7% malformed larvae. Cold shock initiated at 5 min PA for 120 min resulting in 100% triploidy and a hatching rate of 20.2%, malformed larvae accounted for 5.5% of larvae was considered as the most effective treatment for inducing 100% triploidy in pikeperch. |
| Databáze: | OpenAIRE |
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