RNA Isolation from Adipose Tissue: An Optimized Procedure for High RNA Yield and Integrity

Autor:	Karsten Hemmrich, Diego Hoffmeister, Norbert Pallua, Bernd Denecke, Nora E. Paul
Rok vydání:	2010
Předmět:	Yield (chemistry) Biochemistry (medical) Clinical Biochemistry Adipose tissue RNA Body region Rna degradation RNA stabilization RNA extraction Subcutaneous adipose tissue Biology Molecular biology
Zdroj:	Laboratory Medicine. 41:104-106
ISSN:	1943-7730 0007-5027
DOI:	10.1309/lmfsbpuoa19mh5bv
Popis:	Adipose tissue physiology strongly differs between body regions. Reasonable amounts of intact RNA are required to analyze the local differences on a molecular level. Therefore, we developed an optimized RNA isolation procedure from adipose tissue samples. Excised human subcutaneous adipose tissue was obtained from elective operations, and the RNA Stabilization Reagent (RNAlater) was tested for its effect on RNA integrity. Additionally, 3 different RNA isolation kits were evaluated for efficacy in isolating RNA from tissue samples. The samples displayed a significant loss in recoverable RNA and signs of RNA degradation 30 to 60 minutes after tissue excision. The application of RNAlater significantly delayed this degradation. Using the RNeasy Lipid Tissue Kit resulted in a significantly higher RNA yield compared to the RNeasy Mini Kit. Our results demonstrate that combining the RNAlater and RNeasy Lipid Tissue Kit results in a higher yield of RNA even from relatively small tissue samples with guaranteed RNA integrity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::26fbad94a4f6af8531181157279f1315 https://doi.org/10.1309/lmfsbpuoa19mh5bv Zobrazit plný text záznamu