| Autor: |
Wei Dai, Yan Lin, Sheng Gao, Peng Cheng Bi, Jin Hua He, Shi Yun Li |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Journal of Biomaterials and Tissue Engineering. 12:1870-1877 |
| ISSN: |
2157-9083 |
| DOI: |
10.1166/jbt.2022.2617 |
| Popis: |
Objective: To investigate: (1) the role of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in neuroblastoma cell proliferation and; (2) molecular mechanisms underlying the effect. Methods: The cell lines SH-SY5Y, SK-N-SH, and LA-N-5 (neuroblastomas) and the cell line HEK-293 (embryonic kidney) were cultured in vitro. We performed reverse transcription polymerase chain reaction to verify relative gene expressions; WST-8 assay to determine cell proliferation; colony formation assay to analyze cell clonogenic capacity; and western blotting to assess relative protein expressions. Dual-fluorescent reporters were constructed to verify gene–gene interactions. Results: MALAT1 expression was highest in LA-N-5. Decreased MALAT1 expression in LA-N-5 cells effectively inhibited proliferation, increased microRNA (miR)-1297 expression, and decreased E2F transcription factor 7 (E2F7) expression. MALAT1 bound to miR-1297, which then bound to E2F7–both in a targeted manner. Conclusion: MALAT1 acts as an miR-1297 sponge, abolishing the inhibitory actions of miR-1297 on E2F7 translation and ultimately affecting neuroblastoma cell proliferation. Our findings are consistent with the potential of targeting MALAT1 in neuroblastoma treatment. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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