Determining Sequence Fidelity in Repeating Sequence Poly(lactic-co-glycolic acid)s
| Autor: | Han H. Liu, Michael A. Washington, Joseph A. Giesen, Tara Y. Meyer, Ryan M. Weiss, Scott M. Grayson, Jian Li |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
1h nmr spectroscopy Polymers and Plastics Chemistry Stereochemistry media_common.quotation_subject Organic Chemistry Fidelity 02 engineering and technology Polymer 010402 general chemistry 021001 nanoscience & nanotechnology Mass spectrometry 01 natural sciences 0104 chemical sciences Inorganic Chemistry chemistry.chemical_compound Characterization methods Materials Chemistry Copolymer 0210 nano-technology Glycolic acid media_common Sequence (medicine) |
| Zdroj: | Macromolecules. 50:550-560 |
| ISSN: | 1520-5835 0024-9297 |
| DOI: | 10.1021/acs.macromol.6b02202 |
| Popis: | The synthesis and characterization of sequenced copolymers is a relatively underdeveloped field despite the fact that biological polymer properties are known to depend directly on sequence. The concept of sequence fidelity is introduced and defined for repeating sequence copolymers as the ratio of repeat units of the target sequence to the total number of repeat units. 1H NMR spectroscopy and matrix-assisted laser desorption/ionization time-of- flight mass spectrometry (MALDI–ToF MS) are used to characterize the sequence fidelity of repeating sequenced copolymers comprising poly(lactic-co-glycolic acid)s. Both characterization methods are found to have both advantages and weaknesses with regard to identifying and quantifying error in these copolymers. A method for using routine MALDI–ToF MS data to quantify sequence fidelity is proposed and applied to a series of copolymers with synthetically defined error rates. The MS-determined sequence fidelities are comparable to those obtained by NMR analysis. |
| Databáze: | OpenAIRE |
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