A nanostructured SAW chip-based biosensor detecting cancer cells
| Autor: | Klaus Lücke, Markus Perpeet, T.M.A. Gronewold, Patrick Bröker |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
biology
Chemistry Regeneration (biology) Metals and Alloys Nanotechnology Condensed Matter Physics Chip Surfaces Coatings and Films Electronic Optical and Magnetic Materials Circulating tumor cell Cell culture Cancer cell Materials Chemistry Biophysics biology.protein Electrical and Electronic Engineering Antibody Instrumentation Biosensor Human cancer |
| Zdroj: | Sensors and Actuators B: Chemical. 165:1-6 |
| ISSN: | 0925-4005 |
| Popis: | A nanostructured chip surface was fabricated enabling binding via spaced antibodies specifically targeting surface proteins of cancer cells and detection of extremely low numbers of circulating tumor cells (CTC) without labeling using a sam ® 5 biosensor. The antibody surfaces mostly were generated by self assembly of antibodies to gold nanospots on the sensitive SiO 2 -surface of a sam ® 5 chip. Compared with a complete gold surface, only 40% of the amount of antibodies was bound to the nanospot surface, but structured such that 15-fold higher sensitivity to vital cancer cells was achieved. Human cancer cell lines JEG-3 (lymphoblastic leukemia) and MOLT-17 (placental choriocarcinoma) from cell cultures were successfully detected. The sensor showed significant responses on less than 10 cells injected in a single run. The extreme increase in sensitivity and its simple regeneration emphasizes the usefulness of its introduction in biomedical applications. |
| Databáze: | OpenAIRE |
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