Comparative efficacy of cryoprotectant in the lyophilizationof pigeon paramyxovirus-1 vaccine
| Autor: | C. Guha, A. Chatterjee, U. Biswas, D.P. Isore, Mousam Das |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Veterinary medicine Sucrose General Veterinary Hemagglutination Cryoprotectant Fowl Embryonated Biology biology.organism_classification Virology Virus 03 medical and health sciences chemistry.chemical_compound Titer 030104 developmental biology chemistry Animal Science and Zoology Specific-pathogen-free |
| Zdroj: | Indian Journal of Animal Research. |
| ISSN: | 0976-0555 0367-6722 |
| DOI: | 10.18805/ijar.v0iof.7656 |
| Popis: | Preparation of live attenuated freeze dried Pigeon Paramyxo Virus-1 (PPMV-1) vaccine selection of proper cryoprotectant which could preserve and protect the virus against injury due to reduction of temperature at the time of lyophilization, was very necessary. PPMV-1 locally isolated lentogenic strain was selected as seed virus which was properly attenuated by serially passaging in 9 to 10 days old embryonated specific pathogen free (SPF) fowl eggs and produced master seed virus and working seed virus. Five per cent lactalbumin hydrolysate with 10% sucrose (LAS) and 1% polyvinyl pyrrolidone (PVP) were used as two different cryoprotectant for lyophilization of working seed virus. After lyophilization value of haemagglutination (HA) titer and embryo infective dose fifty per ml (EID50/ml) were changed and concluded that both LAS and PVP could be used as suitable cryoprotectant where LAS was better than PVP which was first time done in the whole world. |
| Databáze: | OpenAIRE |
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