Changes in Morphology, Physiology, Metabolism, and Fertilization Capacity of Rainbow Trout Semen following Cryopreservation
| Autor: | B. Berger, T. Weismann, Franz Lahnsteiner, R. A. Patzner |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
endocrine system
medicine.medical_specialty urogenital system Physiology Motility Semen Metabolism Biology urologic and male genital diseases Cryopreservation Motile spermatozoa fluids and secretions Human fertilization Endocrinology Internal medicine medicine Rainbow trout General Agricultural and Biological Sciences reproductive and urinary physiology Pyruvate kinase |
| Zdroj: | The Progressive Fish-Culturist. 58:149-159 |
| ISSN: | 1548-8640 0033-0779 |
| DOI: | 10.1577/1548-8640(1996)058<0149:cimpma>2.3.co;2 |
| Popis: | Alterations in morphology, physiology, metabolism, and fertilization capacity of the semen of rainbow trout Oncorhynchus mykiss after cryopreservation were investigated. In frozen–thawed semen, only about 10–20% of the spermatozoa had an unchanged structure that was similar to untreated spermatozoa; about 20–40% showed intensive signs of swelling of the head and midpiece regions and of the mitochondria. The remaining spermatozoa were damaged in some way. When compared with spermatozoa in untreated semen, the percentage of immotile spermatozoa in frozen–thawed semen was significantly higher, and the rate of motile spermatozoa was decreased. In fertilization solution, the motility of spermatozoa was mainly circular before cryopreservation and linear after cryopreservation. The swimming velocity of spermatozoa did not differ between untreated and frozen–thawed semen. Activities of adenylate kinase and of pyruvate kinase in untreated semen and frozen–thawed semen did not differ significantly. Activit... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text