A novel approach to the analysis of the initiation of embryo development in gramineae
| Autor: | Ingo Schubert, H.-M. Meyer, H.-J. Balzer, F. Matzk, Helmut Bäumlein |
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| Rok vydání: | 1995 |
| Předmět: | |
| Zdroj: | Sexual Plant Reproduction. 8 |
| ISSN: | 1432-2145 0934-0882 |
| Popis: | An in vivo model system to study the initiation of embryo development is presented. From the so-called Salmon system of wheat (alloplasmic lines with a 1BL-1RS chromosome translocation), three completely isogenic and homozygous lines were produced by selection for uniformity in about 20 selfing/backcross generations as well as between sublines of doubled haploids. The line (aestivum)-Salmon is male fertile and sexual. The lines (caudata)-Salmon and (kotschyi)-Salmon are male sterile and have a parthenogenetic capacity of about 90%. The expression of nuclear-cytoplasmic male sterility is different for the two parthenogenetic lines. The initiation of autonomous embryo development at defined developmental stages of the ovaries and the maximum degree of parthenogenesis are identical in both parthenogenetic lines as proved by the auxin test and progeny analyses. The protein patterns from ovary extracts of the three isogenic lines were identical for more than 200 spots of 2-D polyacrylamide gels, confirming their homogeneity. However, one protein (P 115.1) was found 3 days before and during anthesis only in ovaries of the parthenogenetic lines. It seems to be involved in the initiation of parthenogenesis. |
| Databáze: | OpenAIRE |
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