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Summary In Dinoflagellate protists, the centrosome is localized close to the nucleus and surrounded by a complex Golgi apparatus as shown in the two phylogenetically distant species, Prorocentrum micans and Crypthecodinium cohnii . Dinoflagellate centrosomes have been identified previously by using anti-β-tubulin antibody and CTR 210, an antibody raised against human cell centrosomes. Cryofixation and cryofracture techniques enabled us to describe the ultrastructure of these centrosome regions which are devoid of classical centriolar structures. We also describe centrosome associated proteins in dinoflagellates and their behaviour during the cell cycle: p80, a nuclear and cytoplasmic protein recently characterized, myosin II antigens and γ-tubulin. We review also the previously described dinoflagellate centrosome associated proteins: β-tubulin, CTR 210 antigens, p72 (HSP 70), α-actin and p56cdc13, a homologue of Schizosaccharomyces pombe cyclin B. The functions of these proteins and their interactions in the centrosome of dinoflagellates are discussed. |
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