Characterization of twenty-four polymorphic microsatellite loci of Rasbora borapetensis

Autor:	Kazuya Nishida, Noriyuki Koizumi, Koichi Shibukawa, Keiji Watabe, Takeshi Takemura, Atsushi Mori, Bounsong Vongvichith, Shinsuke Morioka
Rok vydání:	2013
Předmět:	Genetics Linkage disequilibrium Genetic diversity Biology biology.organism_classification Null allele law.invention Loss of heterozygosity law Microsatellite Allele Ecology Evolution Behavior and Systematics Polymerase chain reaction Rasbora borapetensis
Zdroj:	Conservation Genetics Resources. 5:711-713
ISSN:	1877-7260 1877-7252
DOI:	10.1007/s12686-013-9888-6
Popis:	Twenty-four microsatellite loci were isolated and characterized from the genome of Rasbora borapetensis. Flanking polymerase chain reaction primers were designed and used to amplify these loci in 32 individuals. All loci were polymorphic with allele numbers ranging from 2 to 27, observed heterozygosity from 0.031 to 1.000 and expected heterozygosity from 0.031 to 0.965. All loci conformed to the Hardy–Weinberg equilibrium and no evidence of null alleles was observed. Pairwise comparisons between alleles did not detect any linkage disequilibrium. The high level of polymorphisms observed in these microsatellite loci will enhance future investigations on the genetic differentiation and structure of populations of Rasbora borapetensis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::26b8b890e33f15bba11e53dc6396813b https://doi.org/10.1007/s12686-013-9888-6 Zobrazit plný text záznamu Full text from SpringerLink