Identification of polyketide synthase gene clusters in a phage P1-derived artificial chromosome library of a Philippine strain of Streptomyces sp. PCS3-D2
| Autor: | Aileen B. Custodio, Edwin P. Alcantara |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Genetics 0303 health sciences Strain (chemistry) biology P1-derived artificial chromosome biology.organism_classification 01 natural sciences Streptomyces 03 medical and health sciences 010608 biotechnology Polyketide synthase polycyclic compounds biology.protein Identification (biology) Molecular Biology Gene 030304 developmental biology Biotechnology |
| Zdroj: | Asia Pacific Journal of Molecular Biology and Biotechnology. :56-63 |
| ISSN: | 2672-7277 |
| DOI: | 10.35118/apjmbb.2019.027.2.08 |
| Popis: | A phage P1-derived artificial chromosome (PAC) library was constructed from genomic DNA of Streptomyces sp. PCS3-D2. Polymerase chain reaction (PCR) screening of the PAC library revealed two clones, PAC16D and P222O, which were positively identified to harbor polyketide synthase (PKS) Type I and PKS Type III gene clusters, respectively. Restriction enzyme digestion showed that PAC16D and PAC222O contained a 130 kb and a 140 kb insert, respectively. Results of sequencing and bioinformatics analyses revealed that PAC16D comprised of a full-length PKS type I bafilomycin gene cluster while PAC222O harbored truncated siderophore and putative gene clusters as well as a complete PKS III biosynthetic gene cluster. The PKS III gene cluster had three genes similar to alkyl resorcinol biosynthetic genes, however majority of the novel gene cluster had little similarity to known PKS Type III gene clusters. The successful cloning and identification of these gene clusters from Streptomyces sp. PCS3-D2 serve as the jump off point to further genetic manipulation in order to produce the insecticidal natural product in a heterologous host. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|