IL-2 production in human memory CD8+ T cells depends on ERK1 expression (150.7)
| Autor: | Tae Kon Kim, Cara Benjamin, Qing Ma, Wieder Eric, Krishna Komanduri |
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| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 186:150.7-150.7 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.186.supp.150.7 |
| Popis: | Recently, we showed that human late memory (CD45RA+CD27-) CD8+ T cells have a unique cytokine signature characterized by the lack of IL-2 production, and no IL-2/IFNγ co-production. Prior studies have suggested the importance of ERK1/2, in IL-2 production; however, little is known about the individual contributions of ERK isoforms to the functional signatures of human T cell subsets. We developed flow cytometric methods to study the differential expression of ERK1 and ERK2 in CD8+ T cell maturation subsets in association with their cytokine signatures. We found that the expression of total ERK1, but not ERK2 nor Ras/Raf/MEK1/MEK2, are diminished by ~80% (measured by flow cytometry and western blotting) in late memory CD8+ T cells, in comparison to early memory CD8+ T cells. Consequently, the diminished expression of total ERK1 resulted in decreased total and phosphorylated ERK1/2. In addition, total ERK1 expression in CD8+ T cells was bimodal on flow cytometry (ERK1high and ERK1low) and consistently identified the subset of memory CD8+ T cells capable (ERK1high) or incapable of IL-2 production (ERK1low) (mean IL-2 production in ERK1high vs. in ERK1low: 20% vs. 1.5%, (n=9, p=0.0039)). Interestingly, late memory cells transduced with lentivirus-expressing ERK1 cDNA, produced 2.5 times more IL-2 than control (n=3, p |
| Databáze: | OpenAIRE |
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