Rapid purification of ?-lytic protease and its mutants from small cultures of recombinant Escherichia coli
| Autor: | Robert George Whittaker, Kevin D. Haggett, Lloyd D. Graham |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
chemistry.chemical_classification
Gel electrophoresis Protease medicine.medical_treatment Mutant Biology medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Molecular biology Enterobacteriaceae law.invention Enzyme chemistry Lytic cycle law medicine Recombinant DNA Escherichia coli |
| Zdroj: | Biotechnology Techniques. 8:203-208 |
| ISSN: | 1573-6784 0951-208X |
| Popis: | A single-step procedure is described for purifying α-lytic protease and its mutants on a small scale (approx. 5mg) from cell-free supernatants of recombinant E. coli. Although free of non-enzyme protein, these preparations often contain fragments that arise from enzymatic self-digestion. Two strong cation exchange matrices are shown to vary greatly in their ability to separate such degradation products from intact enzyme. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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