Isolation of Atypical Genotype Actinobacillus pleuropneumoniae Serotype 6 in Japan

Autor:	Hiroya Ito, Kiyohito Katsuragi, Shunsuke Akama, Hirofumi Yuzawa
Rok vydání:	2014
Předmět:	Serotype General Veterinary Strain (biology) Multiplex polymerase chain reaction Genotype Nucleic acid sequence Biology Isolation (microbiology) biology.organism_classification Virology Actinobacillus pleuropneumoniae Gene Microbiology
Zdroj:	Journal of Veterinary Medical Science. 76:601-604
ISSN:	1347-7439 0916-7250
DOI:	10.1292/jvms.13-0245
Popis:	We describe here isolation of genetically atypical serotype 6 Actinobacillus pleuropneumoniae in Japan indistinguishable by the multiplex PCR that can discriminate between immunologically cross-reactive serotypes 3, 6 and 8. Nucleotide sequence analysis of capsular export and biosynthesis genes revealed that the atypical isolates have capsular polysaccharide export and synthesis gene sequences that are distinct from those of the serotype 6 reference strain. The atypical strains contain a sequence that is identical with both serotype 3- and 6-specific primers, which causes cross-reactions in multiplex PCR.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::26a9d8ff8be0598e01a49fb98f9d120a https://doi.org/10.1292/jvms.13-0245 Zobrazit plný text záznamu