| Autor: |
Katrin Hübscher, Eirini Trompouki, Danilo Guerini, Roger Geiger, Ole K. Greiner-Tollersrud, Honnappa Srinivas, Sebastian Baasch, Eva Bartok, Hilmar Ebersbach, Salvatore Raieli, Jan Ole Olsen, Martine Marchant, Bodo Grimbacher, Vincent Boehler, Georg Kochs, Aikaterini Polyzou, Regina Link, Cristian R. Smulski, Gunther Hartmann, Máté Krausz, Michele Proietti, Dieter Staab, Maximilian Seidl, Johanna Schepp, Philipp Henneke, Max Warncke |
| Rok vydání: |
2020 |
| Předmět: |
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| DOI: |
10.1101/2020.06.21.162990 |
| Popis: |
Deficiency of adenosine deaminase 2 (DADA2) is a severe, congenital syndrome, which manifests with hematologic, immunologic and inflammatory pathologies. DADA2 is caused by biallelic mutations in ADA2, but the function of ADA2, and the mechanistic link between ADA2 deficiency and the severe inflammatory phenotype remains unclear. Here, we show that monocyte-derived proteomes from DADA2 patients are highly enriched in interferon response proteins. Using immunohistochemistry and detailed glycan analysis we demonstrate that ADA2 is post-translationally modified for sorting to the lysosomes. At acidic, lysosomal pH, ADA2 acts as a novel DNase that degrades cGAS/Sting-activating ligands. Furthermore, we define a clear structure-function relationship for this acidic DNase activity. Deletion of ADA2 increased the production of cGAMP and type I interferons upon exposure to dsDNA, which was reverted by ADA2 overexpression or deletion of STING. Our results identify a new level of control in the nucleic acid sensing machinery and provide a mechanistic explanation for the pathophysiology of autoinflammation in DADA2.One Sentence SummaryADA2 is a lysosomal nuclease controlling nucleic acid sensing and type I interferon production. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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