An Investigation of Polymorphism in SMO and LMF1 Genes and Their Association with Body Size in White Fulani and Muturu Cattle Breeds
| Autor: | Ibrahim Shu’aibu, Matthew J. Hegarty, Ridwan O. Ahmed, Semiu F. Bello |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Advances in Bioscience and Biotechnology. 11:319-344 |
| ISSN: | 2156-8502 2156-8456 |
| Popis: | Cattle production is integral to the people of Africa and her economy. To improve cattle productivity, there is a need to inculcate molecular marker-assisted selection into current breeding practices. In this study, we investigated, in White Fulani and Muturu cattle breeds, polymorphism of two candidate genes (Smoothened—SMO and Lipase Maturation Factor 1—LMF1) that have been reported to influence body size in cattle. The SMO gene encodes for the SMO (smoothened) receptor protein; a membrane signal transducer that plays a role in the control of bone formation and adipogenesis. LMF1 encodes for the LMF1 protein which regulates post-translational maturation of vascular lipases. Desired regions of the genes were amplified and genotyped by direct DNA sequencing or Polymerase chain reaction—Restriction Fragment Length Polymorphism. For association study, linear body measurements traits (BMTs) that can be used to predict body size were measured on each animal and values of BMTs measured were compared between both breeds. Very significant (P SMO while LMF1 was monomorphic at the expected (T > C) exon 4 mutation site. Association analysis revealed that polymorphism on G21234C, C22424T, T22939C and T23458G loci significantly affected BMTs (P SMO gene could be a useful candidate gene to facilitate marker assisted selection for body size while LMF1 is monomorphic in the cattle breeds. |
| Databáze: | OpenAIRE |
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