Increasing efficiency of laser fluorescence diagnostics of microbial diseases
| Autor: | M. V. Makarova, V. A. Khomenko, M. T. Alexandrov, Pashkov Ep, E. N. Vasiliev, G. P. Kuzmin, O. G. Gaponenko, G. M. Sorokoumova, Ivanova Ma |
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| Rok vydání: | 2007 |
| Předmět: |
Chemistry
business.industry Laser fluorescence Substrate (chemistry) Condensed Matter Physics Diagnostic system Laser Fluorescence Fluorescence spectra Industrial and Manufacturing Engineering Atomic and Molecular Physics and Optics law.invention Fluorescence intensity Optics law Principal component analysis Biological system business Instrumentation |
| Zdroj: | Laser Physics. 17:1416-1423 |
| ISSN: | 1555-6611 1054-660X |
| Popis: | The laser-induced fluorescence spectra of five types of mycobacteria are studied in various associations. The results obtained are used to demonstrate the possibility of a diagnostic system for the differential diagnostics of microbial diseases. The method employed for the specific analysis of microbial species in the substrate that contain various associations is based on a regression of the spectral characteristics of the fluorescence intensity to the principal components. The diagnostic efficiency is increased when the fluorescence spectra are reconstructed with regard to the optical characteristics of the medium. The detergent effect on the parameters of microbial fluorescence diagnostics is studied. |
| Databáze: | OpenAIRE |
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