Mass production of HzSNPV baculoviruses in immobilizedHeliothis zea (HzAM1) insect cell culture

Autor:	Jeong Hwa Son, Rainer Buchholz, Sung-Koo Kim
Rok vydání:	2004
Předmět:	Insect cell biology Biomedical Engineering Heliothis zea Insect cell culture Nuclear Polyhedrosis Virus Bioengineering biology.organism_classification Applied Microbiology and Biotechnology Virology Inclusion bodies Microbiology Microsphere Cell density Biotechnology
Zdroj:	Biotechnology and Bioprocess Engineering. 9:352-355
ISSN:	1976-3816 1226-8372
DOI:	10.1007/bf02933056
Popis:	Heliothis zea (HzAM1) insect cells were immobilized in microspheres by sodium-cellulosesulfate (NaCS) and polydiallyldimethylammoniumchloride (PDADMAC). The highest HzAMI cell density was 7.5×107 cells/mL in the microspheres. After infection of the immobilized cells byHeliothis zea single nuclear polyhedrosis virus (HzSNPV), the highest concentration of HzSNPV (polyhedral inclusion bodies: PIBs) produced was 2.87×1010 PIBs/mL in the microspheres.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2654b87bcbeedf7d77ef7dbab1d36128 https://doi.org/10.1007/bf02933056 Zobrazit plný text záznamu Full text from SpringerLink