Autor:	Dallan Young, Maurice M. Moloney, Michel Castroviejo, Nancy-Ann Markley, Patricia Laquel
Rok vydání:	1997
Předmět:	biology DNA polymerase DNA replication food and beverages Plant Science General Medicine Processivity DNA polymerase delta Molecular biology RFC2 Proliferating cell nuclear antigen Complementary DNA Genetics biology.protein Agronomy and Crop Science Gene
Zdroj:	Plant Molecular Biology. 34:693-700
ISSN:	0167-4412
DOI:	10.1023/a:1005817220344
Popis:	A cDNA encoding the proliferating cell nuclear antigen (PCNA) from Brassica napus (oilseed rape) was shown to complement the lethal deletion mutation in the PCNA gene (ΔPOL30) of Saccharomyces cerevisiae. We provide unequivocal evidence that the B. napus PCNA can perform all the essential functions of the yeast PCNA in DNA replication, although some species-specific differences may exist. In addition, the B. napus PCNA expressed as a fusion polypeptide with glutathione S-transferase (GST) was shown to stimulate the activity and processivity of two δ-like DNA polymerases from wheat in vitro. These experiments provide direct biochemical evidence that the B. napus PCNA may function as an auxiliary factor in plant cell DNA replication.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2642f981ed3aff9a318411d65b6ede51 https://doi.org/10.1023/a:1005817220344 Zobrazit plný text záznamu Full text from SpringerLink