Effect of freezing on cord blood serum proteins

Autor: V. I. Grishchenko, E. D. Rozanova, A. V. Zinchenko, Eduard O. Nardid, O. A. Nardid, L. V. Tsymbal
Rok vydání: 2009
Předmět:
Zdroj: Biophysics. 54:621-625
ISSN: 1555-6654
0006-3509
DOI: 10.1134/s0006350909050121
Popis: The effect of freezing regimes and storage temperatures on protein conformation and the spectrum of cord blood serum was investigated. Changes in the parameters of EPR spectra of spin probes in cord blood serum after slow freezing and subsequent thawing were established, indicating protein conformational changes characterized by loosening. This fact was confirmed by an earlier, first stage of albumin heat denaturation, as indicated by calorimetric data. It was shown that slow cooling resulted in aggregation of serum protein in which serum albumin and immunoglobulins played an important role. It was concluded that, for retaining the properties of cord blood serum proteins, it is preferable to perform cooling at a rate not lower than 100°C/min and a storage temperature of −80°C and lower.
Databáze: OpenAIRE
Externí odkaz: