Novel Myh11 Dual Reporter Mouse Model Provides Definitive Labeling and Identification of Smooth Muscle Cells—Brief Report
| Autor: | Xianghu Qu, Deqiang Li, Chen-Leng Cai, Megan Sweet, Fuxue Chen, Xiangqin He, Donghua Hu, Jiliang Zhou, Fan Yang, Christina Sanchez, Weinian Shou, Lu Zhang, Kunzhe Dong, Jian Ruan, Jian Shen |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Vascular wall biology Chemistry Neural crest 030204 cardiovascular system & hematology Cell biology Extracellular matrix 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Histone Smooth muscle Gene knockin Myosin biology.protein MYH11 Cardiology and Cardiovascular Medicine |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 41:815-821 |
| ISSN: | 1524-4636 1079-5642 |
| Popis: | Objective: Myh11 encodes a myosin heavy chain protein that is specifically expressed in smooth muscle cells (SMCs) and is important for maintaining vascular wall stability. The goal of this study is to generate a Myh11 dual reporter mouse line for definitive visualization of MYH11 + SMCs in vivo. Approach and Results: We generated a Myh11 knock-in mouse model by inserting LoxP-nlacZ-4XpolyA-LoxP-H2B-GFP-polyA-FRT-Neo-FRT reporter cassette into the Myh11 gene locus. The nuclear (n) lacZ-4XpolyA cassette is flanked by 2 LoxP sites followed by H2B-GFP (histone 2B fused green fluorescent protein). Upon Cre-mediated recombination, nlacZ-stop cassette is removed thereby permitting nucleus localized H2B-GFP expression. Expression of the nuclear localized lacZ or H2B-GFP is under control of the endogenous Myh11 promoter. Nuclear lacZ was expressed specifically in SMCs at embryonic and adult stages. Following germline Cre-mediated deletion of nuclear lacZ, H2B-GFP was specifically expressed in the nuclei of SMCs. Comparison of nuclear lacZ expression with Wnt1 Cre and Mef2c Cre mediated-H2B-GFP expression revealed heterogenous origins of SMCs from neural crest and second heart field in the great arteries and coronary vessels adjacent to aortic root. Conclusions: The Myh11 knock-in dual reporter mouse model offers an exceptional genetic tool to visualize and trace the origins of SMCs in mice. |
| Databáze: | OpenAIRE |
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