Effect of ferritin overexpression in tobacco on the structure of bacterial and pseudomonad communities associated with the roots

Autor: Gérard Vansuyt, Philippe Lemanceau, Agnès Robin, Sylvie Mazurier, Christophe Mougel, Séverine Siblot
Rok vydání: 2006
Předmět:
Zdroj: FEMS Microbiology Ecology. 58:492-502
ISSN: 1574-6941
0168-6496
DOI: 10.1111/j.1574-6941.2006.00174.x
Popis: The genetic structures of total bacterial and pseudomonad communities were characterized in rhizosphere soil and rhizoplane+root tissues of tobacco wild type and a ferritin overexpressor transgenic line (P6) by a cultivation-independent method using directly extracted DNA at the end of three consecutive plant cultures. The structure of total bacterial communities was characterized by automated ribosomal intergenic spacer analysis (A-RISA), and that of pseudomonad communities was characterized by PCR-restriction fragment length polymorphism (PCR-RFLP) from DNA amplified with specific primers. The structure of total bacterial communities was significantly modified in the rhizosphere soil by the overaccumulation of iron in the tobacco transgenic P6 line at the first culture, to a lesser extent at the second culture, and not at all at the third culture. No significant difference was recorded between the total communities associated with the roots (rhizoplane+root tissues) of the two plant genotypes in any of the cultures. In contrast, the difference in pseudomonad structure between the two plant genotypes increased with successive culture at the root level, but was not detected at a significant level in the rhizosphere soil. The impact of iron overaccumulation by the tobacco transgenic P6 line on pseudomonads supports previous findings on the importance of iron competition among fluorescent pseudomonads.
Databáze: OpenAIRE
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