An enzyme-linked immunosorbent assay to quantify 14-3-3 proteins in the cerebrospinal fluid of suspected Creutzfeldt-Jakob disease patients
| Autor: | C. Brechtel, C. J. Gibbs, Hidehiro Takahashi, Kimbra Kenney, Pat Anderson, K. Kurohara |
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| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Pathology medicine.medical_specialty business.industry Disease Elisa assay medicine.disease nervous system diseases Central nervous system disease Enzyme Cerebrospinal fluid Neurology chemistry mental disorders medicine Western immunoblot In patient Neurology (clinical) Prospective cohort study business |
| Zdroj: | Annals of Neurology. 48:395-398 |
| ISSN: | 0364-5134 |
| DOI: | 10.1002/1531-8249(200009)48:3<395::aid-ana18>3.0.co;2-a |
| Popis: | The detection of 14-3-3 protein by Western immunoblot is a sensitive and specific cerebrospinal fluid marker of Creutzfeldt-Jakob disease (CJD). We developed a quantitative enzyme-linked immunosorbent assay (ELISA) that reliably detects 14-3-3 in cerebrospinal fluid. In a prospective study of 147 cerebrospinal fluid samples, the mean 14-3-3 concentration among pathologically confirmed CJD patients (28.0+/-20.6 ng/ml, n = 41) is significantly higher than the mean in the cerebrospinal fluid of those with other neurological disorders (3.1+/-2.9 ng/ ml, n = 84). At a cutoff value of 8.3 ng/ml, the ELISA has a sensitivity of 92.7% and a specificity of 97.6%. The 14-3-3 ELISA supports a diagnosis of CJD in patients who fulfill clinical criteria for possible CJD. |
| Databáze: | OpenAIRE |
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