Simultaneous Determination of Five Irdoid Glycosides and Three Flavonoid Glycosides in Hedyotis Diffusa Wild by UPLC-UV with Ultrasound-Assisted Extraction
| Autor: | Xiaoying Zhang, Bing Chen, Youjia Wu, Hongbin Luo, Xinhua Lin, Peiying Shi, Xuliang Huang, Hong Yao |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Chromatography Flavonoid glycosides biology 010401 analytical chemistry Extraction (chemistry) Biophysics Pharmaceutical Science Glycoside Ultrasound assisted biology.organism_classification 01 natural sciences Biochemistry 0104 chemical sciences Hedyotis diffusa 03 medical and health sciences 0302 clinical medicine chemistry 030220 oncology & carcinogenesis Molecular Medicine |
| Zdroj: | Current Pharmaceutical Analysis. 15:808-818 |
| ISSN: | 1573-4129 |
| DOI: | 10.2174/1573412915666190329220647 |
| Popis: | Background: Hedyotis diffusa is one of the most extensively used Chinese herbs for the treatment of varieties of cancers in the folk of China. The ingredients of the preparation are still not very clear and the quality control method of the herb medicine requires further improvement. Introduction: In this paper, the quantitative methods for the quality control of H. diffusa were originally developed by using UPLC-UV. Methods: Using the five irdoid glycosides and three flavonoid glycosides previously obtained from H. diffusa as index components, referring to 10-Acetyl Scandoside Methyl Ester (ASME), Scandoside (SD), Deacetyl Aspemloside (DA), Asperulosidic Acid (AA), Asperuloside (AD), Qurecetin-3-OSophoroside (QS), Quercetin-3-O-[2-(6-O-E-Sinapoyl-β-D-Glucopyranosyl]-β-D-Glucopyranosyl (QESGG), and Quercetin-3-O-[2-O-(6-O-E-Feruloyl)-β-D-Glucopyranosyl]-β-Glucopyanoside (QEFGG), the extraction conditions, including solvents, durations, and cycles were optimized firstly. Subsequently, a new UPLC-UV method was originally established to simultaneously determine the eight ingredients in the Chinese herbal medicine. Results: The validated method exhibited good linearity (R2 > 0.999), repeatability (RSD < 3.01%), intra- and inter-day precision (RSD Conclusion: The established UPLC-UV method could serve as a rapid and effective tool for quality evaluation of the herbal medicine, H. diffusa. |
| Databáze: | OpenAIRE |
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