Determination of Total Organic Acids in Urine by Extraction with Organic Solvents

Autor:	Ralph B. Dell, Tsai-Tse Yao, Leo Kesner
Rok vydání:	1973
Předmět:	chemistry.chemical_classification Tetramethylammonium hydroxide Chromatography Silica gel Bicarbonate Biochemistry (medical) Clinical Biochemistry Metabolism Phenolphthalein Amino acid Excretion chemistry.chemical_compound chemistry Titration
Zdroj:	Clinical Chemistry. 19:593-596
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/19.6.593
Popis:	Metabolic carboxylic acids are extracted, with a mixture of equal volumes of t-amyl alcohol—chloroform, from an acidified sample of as little as 200 µl of urine that has been adsorbed to a short column of silica gel. Total acid in the extract is measured by titration with tetramethylammonium hydroxide (10 mmol/liter) to a phenolphthalein end point. There is no interference from protein, creatinine, creatine, bicarbonate, or amino acids, and no pretreatment of the sample is required. Results compared well (r = 0.94) with those obtained by the Palmer—Van Slyke method for total organic acids. This procedure should prove useful as a simple, rapid screening test for those metabolic disturbances and inborn errors of metabolism characterized by increased excretion of organic acids.
Databáze:	OpenAIRE
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