| Autor: |
I. E. Liener, E. J. Whitley |
| Rok vydání: |
1975 |
| Předmět: |
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| Zdroj: |
Preparative Biochemistry. 5:433-453 |
| ISSN: |
0032-7484 |
| DOI: |
10.1080/00327487508061590 |
| Popis: |
The construction and operation of an apparatus is described which enables α-antitrypsin to be isolated from human serum by preparative electrophoresis in polyacrylamide gel. The initial pass through the chamber yields a fraction that is predominantly albumin and several α1-proteins. After removal of albumin by affinity chromatography, a second pass through the chamber separates the individual α1-proteins. A volume of 50 ml of serum may be accommodated by the chamber, and the recovery of activity in each step is greater than 60%. The entire procedure may be completed in 36 hrs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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