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Phytochemical curcumin (Cur), a promising dietary supplement for cancer prevention and liver protection, has been shown to induce tumor cell apoptosis (Ao). However, the effect of Cur on PMN Ao and the underlying mechanisms remain unknown. Methods. PMNs (106 cells/ml) freshly isolated from normal volunteers were incubated in vitro in the presence or absence of Cur (10–50 μM). Ao was examined by morphology and TUNEL analyses. The effect of Cur on LPS (100 ng/ml)-induced Ao inhibition was also studied. Myeloidperoxidase (MPO) activity was analyzed to determine the effect of Cur on PMN degranulation. To elucidate the potential mechanism, nuclear NF-kB activity was examined by EMSA. Statistical analysis was performed by ANOVA and Tukey’s test. Results. The data demonstrated that Cur increased PMN Ao from 7.17 ± 0.29 at 0 μM to 28.0 ± 2.0% at 40 μM after 6 h incubation (P < 0.05). LPS-induced Ao suppression was also reversed by Cur (Fig. 1). Download full-size image PMN degranulation was inhibited by Cur as measured by a significant decrease in MPO activities (Table). The inhibition of nuclear NF-kB activity (58% at 1 h and 54% at 2 h) induced by LPS appears to contribute to the pro-apoptotic effect of Cur (Fig. 2). Conclusions. Since the delayed PMN Ao exacerbates acute respiratory distress syndrome and multiple organ failure, the characteristics of curcumin, including pro-apoptotic effects and anti-degranulation potential, make it a potential candidate for prevention and treatment of lung inflammation and injury. TABLE—ABSTRACT 13. Group MPO activity (absorbance ± SD) Medium 0.012 ± 0.002 LPS 0.101 ± 0.021∗ LPS + Cur (10 μ M) 0.103 ± 0.006∗ LPS + Cur (30 μ M) 0.018 ± 0.003# LPS + Cur (50 μ M) 0.014 ± 0.002# (6 h incubation, n = 6, ∗ P < 0.05 versus medium. # P < 0.05 versus LPS alone) |
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