Establishment of a high-frequency regeneration system inCerasus humilis, an important economic shrub
| Autor: | Rui Fang Wang, Feng Lan Huang, Qiu Yan Zhang, Xing Shun Song, Li Na Sun, Jian Zhang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Perennial plant ved/biology fungi ved/biology.organism_classification_rank.species food and beverages Forestry Biology 01 natural sciences Shrub Plant ecology 03 medical and health sciences 030104 developmental biology Murashige and Skoog medium Micropropagation Callus Shoot Botany 010606 plant biology & botany Explant culture |
| Zdroj: | Journal of Forest Research. 21:244-250 |
| ISSN: | 1610-7403 1341-6979 |
| DOI: | 10.1007/s10310-016-0535-4 |
| Popis: | Cerasus humilis is a species of small, perennial, drought-resistant and multipurpose deciduous shrub grown in arid and semi-arid conditions in northern China. In this study, an efficient protocol for the rapid micropropagation of C. humilis has been standardized using stem and/or leaf explants. Direct multiple shoot induction was observed when the stem explants were cultured on Murashige and Skoog (MS) medium supplemented with different plant growth regulators. The highest shoot induction was obtained when stem explants from adult trees were cultured on MS medium supplemented with 2.0 mg L−1 6-benzyladenine (6-BA) and 0.9 mg L−1 α-naphthaleneacetic acid (NAA). The leaf and stem explants cultured on MS medium with 1.0 mg L−1 6-BA and 0.6 mg L−1 NAA, and 0.5 mg L−1 6-BA and 0.8 mg L−1 NAA, respectively, produced the highest induction frequency of callus. Maximum proliferation of callus was observed on MS medium containing a combination of 0.5 mg L−1 6-BA with 0.6 mg L−1 2,4-dichlorophenoxyacetic aci... |
| Databáze: | OpenAIRE |
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