Ordered Coimmobilization of a Multienzyme Cascade System with a Metal Organic Framework in a Membrane: Reduction of CO2 to Methanol
| Autor: | Juan Zhang, Wang Mei, Yanrong Jia, Qin Cunqi, Huagang Ni, Dailian Zhu, Peng Ye, Huihui Deng, Ao Shanshi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
biology Immobilized enzyme Glutamate dehydrogenase 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Formate dehydrogenase 01 natural sciences Cofactor 0104 chemical sciences chemistry.chemical_compound Membrane chemistry biology.protein General Materials Science Methanol 0210 nano-technology Formaldehyde dehydrogenase Nuclear chemistry Alcohol dehydrogenase |
| Zdroj: | ACS Applied Materials & Interfaces. 11:33581-33588 |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | Enzymatic reduction of CO2 is of great significant, which involves an efficient multienzyme cascade system (MECS). In this work, formate dehydrogenase (FDH), glutamate dehydrogenase (GDH), and reduced pyridine nucleotide (NADH) (FDH&GDH&NADH), formaldehyde dehydrogenase (FalDH), GDH, and NADH (FalDH&GDH&NADH), and alcohol dehydrogenase (ADH), GDH, and NADH (ADH&GDH&NADH) were embedded in ZIF-8 (one kind of metal organic framework) to prepare three kinds of enzymes and coenzymes/ZIF-8 nanocomposites. Then by dead-end filtration these nanocomposites were sequentially located in a microporous membrane, which was combined with a pervaporation membrane to timely achieve the separation of product methanol. Incorporation of the pervaporation membrane was helpful to control reaction direction, and the methanol amount increased from 5.8 ± 0.5 to 6.7 ± 0.8 μmol. The reaction efficiency of an immobilized enzymes-ordered distribution in a membrane was higher than that disordered distribution in the membrane, and the methanol amount increased from 6.7 ± 0.8 to 12.6 ± 0.6 μmol. Moreover, it appeared that introduction of NADH into ZIF-8 enhanced the transformation of CO2 to methanol from 12.6 ± 0.6 to 13.4 ± 0.9 μmol. Over 50% of their original productivity was retained after 12 h of use. This method has wide applicability and can be used in other kinds of multienzyme systems. |
| Databáze: | OpenAIRE |
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