Human liver glycogen metabolism assessed with a 13 C-enriched diet and a 13 CO2 breath test
| Autor: | Tanis, Kroneman, Wattimena, Rietveld, van den J Jan Berg, Swart, Nieland |
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| Rok vydání: | 1998 |
| Předmět: |
Breath test
medicine.medical_specialty Glycogenolysis medicine.diagnostic_test Glycogen digestive oral and skin physiology Clinical Biochemistry General Medicine Metabolism Carbohydrate Biochemistry Excretion chemistry.chemical_compound Endocrinology Gluconeogenesis chemistry Glycogenesis Internal medicine medicine |
| Zdroj: | European Journal of Clinical Investigation. 28:466-474 |
| ISSN: | 0014-2972 |
| DOI: | 10.1046/j.1365-2362.1998.00316.x |
| Popis: | Background Adequate liver glycogen stores to maintain hepatic glucose output by glycogenolysis in the post-absorptive state are essential to prevent protein loss through gluconeogenesis. There are no simple techniques to monitor liver glycogen use. Methods In this study, we labelled liver glycogen with naturally 13 C-enriched carbohydrate and measured the pattern of 13 CO 2 excretion and the post-prandial time during which oxidation of 13 C-labelled liver glycogen was demonstrable by 13 CO 2 enrichment in breath. Two experiments were performed in 24 healthy volunteers. Results In the first experiment we observed that breath 13 CO 2 enrichment returned to baseline values at 20.3 (SD 2.3, n = 12) hours post-prandially, indicating exhaustion of the 13 C-labelled liver glycogen at that time. In a second experiment, breath 13 CO 2 enrichment in the early hours of the post-prandial phase was studied. After a steep decline, which started 2-4 h after the last meal, the 13 CO 2 enrichment reached a plateau phase 6 h post-prandially. This plateau phase lasted for about 6-8 h, suggesting steady-state glycogenolysis during this period. The plateau phase was followed by a further decline in 13 CO 2 excretion, suggesting a gradually diminishing contribution of 13 C-labelled liver glycogen to substrate oxidation. Conclusion It is possible to label liver glycogen with a diet of naturally 13 C-enriched carbohydrate. The oxidation of the labelled liver glycogen can be monitored by measuring 13 C-enrichment in breath CO2. |
| Databáze: | OpenAIRE |
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