| Autor: |
Hong Kiu Francis Fok, Yusong Guo, Yang Yang, Tingting Wang, Xin Deng, Mo Wang, Wendi Xiao, Wen-Bin Zhang, Zhongguang Yang, Songzi Kou, Yan Yan, Bojing Jiang, Fei Sun |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Matter. 2:233-249 |
| ISSN: |
2590-2385 |
| DOI: |
10.1016/j.matt.2019.09.013 |
| Popis: |
Summary Design of proteins with nonlinear topologies has emerged as a nascent branch of protein engineering, but significant applications remain to be seen. Here, we demonstrate the cellular synthesis of (SpyCatcher)4GFP, a 4-arm star-like protein enabled by spontaneous split GFP reconstitution, which further led to the creation of various protein networks exhibiting tunable mechanics and suitability for cell encapsulation. A derivative 4-arm star-like protein, (CarHC)4GFP, resulting from the conjugation of (SpyCatcher)4GFP with the SpyTag-fusion CarHC photoreceptors, can undergo rapid sol-gel and gel-sol transitions in response to AdoB12 and light, respectively. The chemo- and photo-induced phase transitions enabled encapsulation and controlled release of protein molecules such as the biofilm-degrading glycosyl hydrolase PslG, a potential agent for combatting multidrug-resistant bacterial species in chronic infections. The creation of those uncommon protein architectures promises great opportunities for materials biology and “smart” therapeutic delivery. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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