A morphological study of the changes in the ultrastructure of a bacterial biofilm disrupted by an ac corona discharge in air
| Autor: | Anatoly Kudryavtsev, Alexander Astafiev, Rybal'chenko Ov, V. V. Kapustina, Olga Stepanova, Orlova Og |
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| Rok vydání: | 2016 |
| Předmět: |
endocrine system
Population Analytical chemistry General Physics and Astronomy 02 engineering and technology medicine.disease_cause 01 natural sciences Corona (optical phenomenon) 0103 physical sciences medicine education Escherichia coli Corona discharge 010302 applied physics education.field_of_study biology Chemistry Biofilm Corona treatment biochemical phenomena metabolism and nutrition 021001 nanoscience & nanotechnology biology.organism_classification Ultrastructure Biophysics 0210 nano-technology Bacteria |
| Zdroj: | Journal of Applied Physics. 120:063302 |
| ISSN: | 1089-7550 0021-8979 |
| DOI: | 10.1063/1.4959992 |
| Popis: | The morphology of bacterial cells and biofilms subjected to a low frequency (∼105 Hz) ac (∼10−1 A) corona discharge was investigated using electron microscopy. A low-frequency ac corona discharge in air is shown to have a bactericidal and bacteriostatic effect on Escherichia coli M17 culture at both the cellular and population levels. Corona exposure inhibits the formation of a microbial community and results in the destruction of formed biofilms. This paper presents data on changes in the ultrastructure of cells and biofilms after corona treatment. Our results suggest that the E. coli M17 cells inside biofilms are affected with results similar to sub-lethal and lethal thermal exposure. Some of the biological aspects of colony and biofilm cells death are evaluated. Morphological changes in the ultrastructure of the biofilms under corona treatment are described. Our results indicate that the heating effect is the main factor responsible for the corona-induced inactivation of bacteria. |
| Databáze: | OpenAIRE |
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