Activation of HIV in Human Skin by Ultraviolet B Radiation and its Inhibition by NFκB Blocking Agents¶
| Autor: | Kiyoshi Ariizumi, Dorothy E. Lewis, Makoto Bonkobara, Joan Breuer-McHam, Eric Simpson, D. Brian Dawson, Ponciano D. Cruz, Madeleine Duvic, Irene Dougherty |
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| Rok vydání: | 2001 |
| Předmět: |
integumentary system
business.industry Human immunodeficiency virus (HIV) Ultraviolet b Human skin General Medicine In situ hybridization Pharmacology medicine.disease_cause Biochemistry Nuclear factor kappa b In vitro Ultraviolet B radiation In vivo Medicine Physical and Theoretical Chemistry business |
| Zdroj: | Photochemistry and Photobiology. 74:805 |
| ISSN: | 0031-8655 |
| DOI: | 10.1562/0031-8655(2001)074<0805:aohihs>2.0.co;2 |
| Popis: | To determine whether ultraviolet B (UVB) irradiation leads to activation of HIV in human skin, we conducted prospective and controlled studies in two academic medical centers in Texas from July 1995 to April 1999. HIV-positive patients with UV-treatable skin diseases were enrolled at each center, 18 subjects at one and 16 at the other. In one center, specimens from lesional and nonlesional skin biopsies were taken before and after sham- or UVB-irradiation administered in vivo or in vitro. In the other center, UVB phototherapy was administered three times weekly and specimens from skin biopsies were taken before and after 2 weeks (six treatments). Cutaneous HIV load was assessed using reverse transcriptase-polymerase chain reaction and reverse transcriptase-polymerase chain reaction in situ hybridization. UVB irradiation led to a 6–10-fold increase in the number of HIV in skin. To ascertain a role for nuclear factor kappa B (NFκB) in UVB-inducible HIV activation, two types of blockers, NFκB oligon... |
| Databáze: | OpenAIRE |
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