Optimization of fecal sample preparation for untargeted LC-HRMS based metabolomics
| Autor: | Nora Cesbron, Alexandre Sydor, B. Le Bizec, G. Dervilly-Pinel, Yann Guitton, Anne-Lise Royer |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Chromatography Serial dilution Endocrinology Diabetes and Metabolism 010401 analytical chemistry Clinical Biochemistry Extraction (chemistry) Context (language use) Biology 01 natural sciences Biochemistry 0104 chemical sciences 03 medical and health sciences 030104 developmental biology Metabolomics Metabolome Sample preparation Animal species Feces |
| Zdroj: | Metabolomics. 13 |
| ISSN: | 1573-3890 1573-3882 |
| DOI: | 10.1007/s11306-017-1233-8 |
| Popis: | Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites. In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted LC-HRMS fingerprinting. The conditions of extraction (quantity, preparation, solvents, dilutions) were investigated in bovine feces. A rapid and simple protocol involving feces extraction with methanol (1/3, M/V) followed by centrifugation and a step filtration (10 kDa) was developed. The workflow generated repeatable and informative fingerprints for robust metabolome characterization. |
| Databáze: | OpenAIRE |
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