Autor: V. N. Kuvshinov, L. S. Sandakhchiev, Alexey Masharsky, Alexander A. Ilyichev, M. Sh. Azaev, Klimov Na, O. Yu. Tumanova, Andrei P. Kozlov
Rok vydání: 2001
Předmět:
Zdroj: Molecular Biology. 35:130-133
ISSN: 0026-8933
Popis: A phage peptide library was screened with virus-neutralizing monoclonal antibodies (MAb) 2F5 which recognize a conserved epitope of HIV-1 gp41. Phages that expose peptides specifically binding with MAb 2F5 were selected by ELISA. Amino acid sequence analysis revealed homology to region 662–671 of HIV-1 HB10 gp160 for most peptides. The major role in recognition was ascribed to Asp-664, Lys-665, and Trp-666. The epitope-mimicking peptides were tested for immunogenicity. Antibodies to gp41 were detected in the serum of immunized rabbits.
Databáze: OpenAIRE
Externí odkaz: