Makisterone C: A 29-carbon ecdysteroid from developing embryos of the cotton stainer bug,Dysdercus fasciatus
| Autor: | James A. Svoboda, W. R. Lusby, Mark F. Feldlaufer, Gunter F. Weirich |
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| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
animal structures Physiology Campesterol Biology Biochemistry chemistry.chemical_compound Internal medicine polycyclic compounds medicine Ecdysteroid Pyrrhocoridae fungi Embryogenesis Embryo General Medicine biology.organism_classification Sterol Titer Endocrinology chemistry Insect Science lipids (amino acids peptides and proteins) hormones hormone substitutes and hormone antagonists Ecdysone |
| Zdroj: | Archives of Insect Biochemistry and Physiology. 18:71-79 |
| ISSN: | 1520-6327 0739-4462 |
| Popis: | An ecdysteroid RIA was used to determine the ecdysteroid titer in developing embryos of Dysdercus fasciatus and revealed that peak titer occurred approximately 120 h post-oviposition. Analysis of neutral sterols at this time indicated sitosterol to be the predominant neutral sterol with lesser amounts of campesterol. Embryonic sterols were highly reflective of the sterols found in the cotton seed diet upon which previous generations of the bugs had fed. Analysis of the embryonic extract for ecdysteroids indicated the presence of both makisterone A and the 29-carbon ecdysteroid makisterone C. Isolation of these compounds was accomplished by reversed-phase and silica HPLC in conjunction with RIA, and the identification of both compounds was confirmed by mass spectrometry. No ecdysone or 20-hydroxyecdysone was detected in the embryonic sample. |
| Databáze: | OpenAIRE |
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