Quantitative Determination of Porphobilinogen in Urine: Reuse of Disposable Ion-Exchange Columns

Autor:	V. Kordač, Jane Serup Pedersen, Torben K. With
Rok vydání:	1981
Předmět:	Chromatography Carrier state Biochemistry (medical) Clinical Biochemistry Urine Porphyria intermittent acute medicine.disease Quantitative determination chemistry.chemical_compound chemistry Porphobilinogen medicine Urine porphobilinogen Acute intermittent porphyria
Zdroj:	Laboratory Medicine. 12:102-105
ISSN:	1943-7730 0007-5027
DOI:	10.1093/labmed/12.2.102
Popis:	It was emphasized in a recent review1 that 1) the determination of urine porphobilinogen (PBG) is the most important analysis in the diagnosis of an attack of acute porphyria, and 2) quantitative ion-exchange analysis for PBG is indispensable in the detection of asymptomatic carriers of acute intermittent porphyria (AIP). Existing methods for quantitative ion-exchange analysis of urine PBG are laborious, timeconsuming and expensive. The simple Bio-Rad PBG test, based on use of disposable resin columns and designed for qualitative assay of PBG, was therefore developed into a quantitative method and modified so that the disposable columns could be reused. This modified test, a feasible and economical technique within the reach of smaller hospital laboratories, is described in detail.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2563ad4aa00f2f53c44af0aa1910e1f5 https://doi.org/10.1093/labmed/12.2.102 Zobrazit plný text záznamu