Development and application of an analytical method for curdione quantification in pregnant sprague-dawley rats by LC-MS/MS

Autor: Juan Jiang, Xu Sichong, Ting Zhang, Ying Li, Liming Chong, Meng Xiang, Zuyue Sun, Yang Yang, Li Zhou, Qi Pan, Yongwei Luo
Rok vydání: 2015
Předmět:
Zdroj: Biomedical Chromatography. 29:1499-1505
ISSN: 0269-3879
DOI: 10.1002/bmc.3449
Popis: The vaginal administration route suffers from relatively low absorption efficiency, which may hinder the identification of the toxicokinetics of curdione in pregnant women. A sensitive analytical method for determining the plasma concentration of curdione was developed and applied in the determination of curdione in pregnant Sprague–Dawley rats as a simulated model. Glimepiride was used as an internal standard and chromatographic separation was achieved on a Capcell Pak C18 MGIII column. A gradient elution profile with 0.5% formic acid (A)–0.5% formic acid–acetonitrile (B) was selected as mobile phase. The selected reaction monitoring mode was used for quantification based on the target fragment ions m/z 237.2 to m/z 135.1 for curdione and m/z 491.3 to m/z 352.1 for the glimepiride. The standard curve was linear over the range of 0.5–500 ng/mL for curdione in rat plasma and yielded a consistent peak pattern, even at the lower limit of quantitation of 0.5 ng/mL. The retention times of curdione and IS were 6.55 and 6.59 min, respectively. The mean recovery of curdione in rat plasma was 95.5–101.1%. The intra-day and inter-day precisions were between 2.35 and 9.08%. This LC-MS/MS method provides a simple and sensitive means for determining the plasma concentration. Copyright © 2015 John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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