Tocotrienol rich fraction supplementation confers protection on the ovary from cyclophasphamide induced apoptosis
| Autor: | Gabriele Ruth Anisah Froemming, Rozzana Mohd Said, Hazar Shakir Salleh, Effat Omar |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
medicine.medical_specialty TUNEL assay Vitamin E medicine.medical_treatment Ovary Biology medicine.disease_cause Andrology chemistry.chemical_compound Infectious Diseases Endocrinology medicine.anatomical_structure chemistry Apoptosis Internal medicine cardiovascular system medicine heterocyclic compounds Tocotrienol Tocopherol Oxidative stress Corn oil |
| Zdroj: | Asian Pacific Journal of Tropical Disease. 4:234 |
| ISSN: | 2222-1808 |
| Popis: | Introduction Cyclophosphamide (CPA) therapy leads to infertility via oxidative stress induced apoptosis of ovarian cells. Tocotrienol, vitamin E isoform, is a much more potent antioxidant compared to tocopherol, the more investigated vitamin E isoform. Tocotrienol rich fraction (TRF) is an affordable and available form of tocotrienol supplementation. The effect of concurrent TRF supplementation with CPA treatment is un-investigated. Objective To study the ovarian protective effect of tocotrienol rich fraction (TRF) when coadministered with CPA. Methods 60 female ICR mice, aged between 8 −10 weeks were divided into 5 treatment groups: CPA, CPA&TRF, normal saline, TRF only, and corn oil (TRF carriage vehicle) only. The treatment was given for 30 consecutive days, after which all animal were euthanized. At dissection, both ovaries were removed, fixed in 10% formalin, processed and embedded in paraffin to form tissue blocks. Terminal deoxynucleotidyl transferase–mediated deoxy-uridine triphosphate nick-end labelling (TUNEL) assay was performed on the tissue blocks. Results & Discussion There was a significant reduction of TUNEL positive cells in the combined CPA&TRF group compared to CPA only group (p Conclusion Co administration of TRF with CPA confers protection against apoptosis in ovaries. TRF is a potential candidate for ovarian conservation in chemotherapy associated damage. |
| Databáze: | OpenAIRE |
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