Axonal transport of BDNF precursor in primary sensory neurons

Autor:	Robert A. Rush, Linda Lin-yan Wu, Hui Wang, Xin-Fu Zhou, Xing-Yun Song, Xue-Gang Luo, Jin-Hua Zhong
Rok vydání:	2006
Předmět:	Brain-derived neurotrophic factor Nerve root Chemistry General Neuroscience Sensory system Spinal cord medicine.disease Cell biology medicine.anatomical_structure nervous system Neurotrophic factors medicine Crush injury Axoplasmic transport Sciatic nerve Neuroscience
Zdroj:	European Journal of Neuroscience. 24:2444-2452
ISSN:	0953-816X
DOI:	10.1111/j.1460-9568.2006.05138.x
Popis:	Recent studies have shown that the precursor of brain-derived neurotrophic factor (pro-BDNF) activates p75NTR with high affinity to induce apoptosis. Here we show that pro-BDNF is transported anterogradely and retrogradely in sensory neurons of adult rats. After a crush injury of sciatic nerves, dorsal roots or dorsal column in adult Sprague-Dawley rats, the immunoreactivity for pro-BDNF accumulated at both the proximal and distal segments. The accumulation reached a maximum at 24 h after injury. Western blot analysis also revealed pro-BDNF in sciatic nerve segments proximal and distal to the ligature and in the spinal cord. Biotinylated or Alexa-488-labelled pro-BDNF injected into sciatic nerve was internalized and transported both retrogradely and anterogradely within sensory neurons. These results demonstrate that pro-BDNF is anterogradely and retrogradely transported in sensory neurons, suggesting that endogenous pro-BDNF may be released and play important functions.
Databáze:	OpenAIRE
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