| Autor: |
Benard Sa, Fowotade Aa, Afolabi Oo, Muhammed Ao, Olutunde Oa |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
African Journal of Cellular Pathology. 4:13-16 |
| ISSN: |
2449-0776 |
| Popis: |
Aim: To investigate the suitability of alcoholic extract of Sorghum bicolor stalk as a counter stain for Hibiscus extract stained nuclei. Method: Formalin fixed kidney and liver biopsies were processed by the paraffin wax technique, sectioned, stained with Hibiscus extract solution and counterstained with 10% alcoholic extract of Sorghum bicolor stalk. Parallel sections were stained with H&E as control. Results: Results show distinct dark - violet nuclear staining with light-brown staining of collagen and other components. Conclusion: Sorghum bicolor stalk alcoholic extract could be a useful substitute to eosin in histology for the demonstration of collagen and other cytoplasmic components especially when H. sabdariffa is used as the nuclear stain. Keywords: Hibiscus, Nuclear staining, Sorghum bicolor extract |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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