Effect of glycerol and PEGMA coating on the efficiency of cell holding in alginate immobilized Synechococcus elongatus
| Autor: | M. del Pilar Sánchez-Saavedra, Ana B. Castro-Ceseña |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Synechococcus elongatus 010604 marine biology & hydrobiology Cell Ether macromolecular substances Plant Science Aquatic Science engineering.material Methacrylate 01 natural sciences chemistry.chemical_compound medicine.anatomical_structure chemistry Chemical engineering Coating Biochemistry 010608 biotechnology medicine engineering Glycerol Liberation Ethylene glycol |
| Zdroj: | Journal of Applied Phycology. 28:63-71 |
| ISSN: | 1573-5176 0921-8971 |
| DOI: | 10.1007/s10811-015-0552-2 |
| Popis: | Synechococcus elongatus cells were immobilized in alginate beads, and the effects of increasing the cross-linker concentration from 2 to 4 % CaCl2 were evaluated, as well as the effects of coating the beads with either glycerol or poly(ethylene glycol) methyl ether methacrylate (PEGMA)—not previously reported for immobilized microalgae—to improve the holding time of the immobilized cells. S. elongatus cells remain metabolically active after coating with glycerol or PEGMA. There is an inverse relation between the glycerol concentration and the chlorophyll a content for the alginate beads cross-linked with 2 % CaCl2. PEGMA diminishes the rate of liberation of cells as its concentration increases, although results suggest the ability of S. elongatus to degrade PEGMA, which increases the growth rate of the liberated cells, because of PEGMA being used as carbon source. |
| Databáze: | OpenAIRE |
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