A Modified Cooling Method and its Application inDrosophilaExperiments
| Autor: | Da-Xiang Yang, Tong-bo Zhu, Wen-hui Qu |
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| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Journal of Biological Education. 49:302-308 |
| ISSN: | 2157-6009 0021-9266 |
| DOI: | 10.1080/00219266.2014.967272 |
| Popis: | Chilling is a cost-effective and safe method of immobilising flies in Drosophila experiments. However, should condensation form on the plate, it would be fatal to the flies. Here we describe a modified cooling method using reusable commercial ice pack(s) (ca. 400 ml, 2–3 cm tall) rather than crushed ice. The ice pack is covered with a piece of filter paper which absorbs the condensed water formed on the cold surface, and the moistened filter paper does not wet the wings of flies inverted onto it. An ice pack (ca. 400 ml) takes effect in at least 1 h, and its height is shorter than the maximum specimen height of a dissecting microscope, so that flies can be checked while they remain on the cold surface. This method overcomes the disadvantages of the indigenous cooling anaesthetising approach and makes it practically feasible. We also describe an easy-to-make fly-transferring toolkit which will greatly facilitate fly transferral when it is combined with our chilling method. In our design, the flies were dir... |
| Databáze: | OpenAIRE |
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