| Popis: |
Coagulase negative staphylococci (CNS) are primary cause of mastitis in ruminant animal, and can either be transmitted by contagious or by environmental means. Therefore, we speculate whether there are similarity in the species distribution of CNS colonsing the anterior nare and milk of ruminant animals that can serve as source of infection. The study examined the species of CNS from the anterior nare of ruminant animals and contact persons. A total of 75 CNS isolates were analysed using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), mecA gene detection by PCR assay and antibiotic susceptibility testing according to CSLI guidelines. Overall, four CNS spp were identified, S.sciuri predominate 71 (96%) and were recovered from all samples analysed, while one specie each (1.3%) of S.lentus, S.condementii and S.xylosus (1.3%) respectively. No mecA gene was detected in the CNS spp identified. The CNS spp demonstrated low to moderate resistance pattern (15-30%) to all antibiotics tested. In conclusion, the study provided a baseline epidemiological information on CNS spp colonizing the ruminants ruminant animal and contact persons. In addition, non-detection of mecA gene and high sensitivity pattern demonstrated by the CNS species. |
