Restriction-Free Construction of a Phage-Presented Very Short Macrocyclic Peptide Library
| Autor: | Michael Hust, Martin Empting, Valentin Jakob, Saskia Helmsing |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cloning chemistry.chemical_classification Phage display 010405 organic chemistry Oligonucleotide Base pair Computer science Peptide Computational biology 01 natural sciences Insert (molecular biology) 0104 chemical sciences 03 medical and health sciences Macrocyclic peptide 030104 developmental biology chemistry Panning (camera) |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493998524 |
| DOI: | 10.1007/978-1-4939-9853-1_6 |
| Popis: | Phage display is a commonly used technology for the screening of large clonal libraries of proteins and peptides. The construction of peptide libraries containing very short sequences, however, poses certain problems for conventional restriction-based cloning procedures, which are rooted in the necessity to purify restricted library oligos. Herein, we present an alternative cloning method especially suitable for such very short sequences of about only 21 base pairs resulting in a 60 bp insert. The employed restriction-free hot fusion cloning strategy allows for facile library construction bypassing the need for purification of the small oligo. The library includes one well-defined disulfide bridge rendering the displayed macrocyclic peptide sequences as attractive scaffolds for novel active principles. |
| Databáze: | OpenAIRE |
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