Orientational imaging and tracking of single CdSe nanocrystals by defocused microscopy
| Autor: | Marie-Virgine Ehrensperger, Maxime Dahan, Xavier Brokmann, Antoine Triller, J.-P. Hermier |
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| Rok vydání: | 2005 |
| Předmět: |
Materials science
Condensed Matter::Other Orientation (computer vision) business.industry Physics::Optics General Physics and Astronomy Nanoparticle Condensed Matter::Mesoscopic Systems and Quantum Hall Effect Tracking (particle physics) Fluorescence Numerical aperture Condensed Matter::Materials Science Dipole Optics Nanocrystal Microscopy Physical and Theoretical Chemistry business |
| Zdroj: | Chemical Physics Letters. 406:210-214 |
| ISSN: | 0009-2614 |
| DOI: | 10.1016/j.cplett.2005.03.007 |
| Popis: | We study the fluorescence patterns produced by single CdSe nanocrystals in defocused microscopy. The patterns are directly related to the structure of the nanocrystal emission dipole and their analysis provides a full determination of the three-dimensional orientation of the nanoparticles. In the case of a high numerical aperture objective, we show that the contour intensities of the defocused patterns can be fitted by a simple analytical formula. Finally, we demonstrate that defocused imaging can be used to track both the position and the orientation of single CdSe nanocrystals coupled to membrane receptors in live cells. |
| Databáze: | OpenAIRE |
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