| Autor: |
Fernando Marcos Rodríguez, Claudia Leonor Carabajal-Miotti, Susana Graciela Ruiz de Frattari, Adriana Haydee Vargas, Natalio Emilio González-Silva, Ivon Teresa Clara Novak |
| Rok vydání: |
2022 |
| Zdroj: |
European Journal of Clinical Medicine. 3:21-29 |
| ISSN: |
2736-5476 |
| DOI: |
10.24018/clinicmed.2022.3.4.225 |
| Popis: |
Background: There are a variety of polymorphonuclear neutrophil phenotypes described in different species and health or disease situations. Objective: Study human neutrophil phenotypes generated in vitro. Methods: Heparinized human blood samples were collected with ethical consent. Polymorphonuclear neutrophils purification and autologous cultures was performed. Neutrophil stimulation was performed with LPS, fMLP or OVA. Immunofluorescence was applied. Results: “Polymorphonuclear neutrophil-antigen presenting cell” profile was generated in vitro, expressing CD80, CD86 and HLA-DR molecules. Immunofluorescence analysis show: CD80 expression, significant differences between CTFT control and CTFT fMLP (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
