Systematic identification and characterization of novel genes in the regulation and biogenesis of photosynthetic machinery

Autor: Moshe Kafri, Weronika Patena, Lance Martin, Lianyong Wang, Gillian Gomer, Arthur K Sirkejyan, Audrey Goh, Alexandra T. Wilson, Sophia E Gavrilenko, Michal Breker, Asael Roichman, Claire D. McWhite, Joshua D. Rabinowitz, Frederick R Cross, Martin Wühr, Martin C. Jonikas
Rok vydání: 2022
DOI: 10.1101/2022.11.12.515357
Popis: SUMMARYPhotosynthesis is central to food production and the Earth’s biogeochemistry, yet the molecular basis for its regulation remains poorly understood. Here, using high-throughput genetics in the model eukaryotic algaChlamydomonas reinhardtii, we identify with high confidence (FDRHighlightsHigh-confidence identification of 70 previously-uncharacterized genes required for photosynthesisProteomic analysis of mutants allows assignment of function to novel genesCharacterization of 5 novel Photosystem I mRNA maturation factors validates this resourceMTF1 and PMR1 identified as master regulators of photosynthesis
Databáze: OpenAIRE